What you will probably find when it is breeding season is that the guinea fowl will pair off and hang about in smaller groups which is usually the groups that they were first raised amongst. All 23 of my guinea fowl now spend the full day together as the breeding season and egg season is over, although I did get one egg today as I kept them in as it was raining heavily.
If your 2 adults were a male and female then they would be one group next breeding season and then the 5 may stick together or split up into a 3 and a 2 depending on the gender. Watch out for this behaviour the pairing up or small groupsing in the Spring and then all of them haging about together again in the Autumn. It is so fascinating to watch.
Interesting I do have quite a few males and although there is a few squaring up and chasing one another around, they do generally get on ok since they are able to free range during the day.
Yes I believe the females would make the two syllable call even if there were no males as they call out to each other regardless of gender.
You should be able to train them to respond to your voice. If I call out "Cooooooome on, cooooooome on!" and I repeat this, the guinea fowl start to head towards the hut when it is about the time that they need to be going in for the night.
Hope all this helps.
Sara @ farmingfriends